This page last changed on Jul 11, 2013 by klimov.

Variables and parameters

Parameter
Notes
Range
 
Cell size range, um
 - detectable 
 - filtration
     
Concentration range, cells/mL      
Detection volume, uL, mL      
Flow rate, mL/min, uL/min
 - per channel cross section

 - per detection cross-section
     
Throughput range, counts/sec. 2E5 (LLNL)      
Signal processing speed (pulses/sec, images/sec)      
Overall energy efficiency; J/count      
Statistics and acceptable errors      
Optical background (Br)      
Detector efficiency (Qr) - determined by laser power, optical design, PMT sensitivity... photoelectrons detected per equivalent fluorochrome molecule      
Linear range (signal : number of photons)      
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